Purification of Recombinant Fusion Polypeptide hEGF-AWRK6 and Effect on Wound Healing and Infection of Burn Model Mice

The study investigated the therapeutic effects of the recombinant fusion polypeptide hEGF-AWRK6 (EK) on burn infections in a mouse model. EK was expressed and purified using an Escherichia coli system. Mice with Ⅱ degree burns and Pseudomonas aeruginosa infections were treated with EK (30 mg/L), while control groups received PBS, gentamicin, or burn ointment. Results showed that EK significantly reduced bacterial colony counts and increased wound healing rates compared to controls (P<0.01). Pathological analysis revealed that EK-treated wounds had more organized dermal cells, increased hair growth, and faster epithelization. These findings suggested that EK could be a promising candidate for developing treatments for burn wounds.