TLDR Using 5-butylpicolinate esters improves the sensitivity and reliability of detecting testosterone and dihydrotestosterone in saliva.
In this study, researchers used proton affinitive derivatization with picolinic acid and its analogs to enhance the sensitivity of detecting testosterone (T) and 5α-dihydrotestosterone (DHT) in saliva samples via LC-ESI-MS/MS. They converted T and DHT into their picolinate esters and analyzed their chromatographic behavior. The esters showed distinct single peaks, with elution order being picolinate, 3/6-methylpicolinate, and 5-butylpicolinate. While the picolinate and 3/6-methylpicolinate esters were significantly affected by saliva matrix interference, the 5-butylpicolinate esters were only slightly impacted, making them more reliable for sensitive determination of T and DHT in saliva.
184 citations,
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April 2022 in “Journal of pharmaceutical and biomedical analysis” The method can measure multiple steroids in human hair to study long-term steroid metabolism, especially in newborns and children.
