Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq

February 2024 in “ STAR Protocols ”

Zishuo Yuan, Junjie Chen, Yanwen Xu, Zhentao Zhou, Pengfei Cai, Xiaoyu Wei, Huiwen Zheng, Jufang Zhang, Yue Yuan, Chuanyu Liu

scalp tissue hair follicles single-cell RNA sequencing scRNA-seq transcriptomics enzymatic digestion cell viability assay

TLDR The document provides a method to prepare human scalp tissue for studying hair follicles at the single-cell level.

The document outlines an optimized protocol for dissociating human scalp tissue to obtain high-quality single-cell suspensions for single-cell RNA sequencing (scRNA-seq), aimed at studying the transcriptomics of human hair follicles. It includes detailed steps for cleaning scalp tissue, removing subcutaneous fat, mechanical mincing, enzymatic digestion, and subsequent procedures for cleaning, resuspending, conducting a cell viability assay, and library construction.

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Research cited in this study

1 / 1 results

research Androgenetic Alopecia: A Review

153 citations , March 2017 in “Endocrine”

Male pattern baldness involves genetics, hormones, and needs better treatments.

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Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

MagicBold in Research 865 upvotes 1 year ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

community So we have 20k dollars human like machines available but no new effective treatment for hairloss since finasteride :)

in Chat 237 upvotes 1 year ago

There have been no new effective hair loss treatments since finasteride, despite technological advancements. Current treatments include finasteride, minoxidil, and RU58841, with ongoing challenges and potential future solutions in research.

community I find it funny a bunch of us on here are on drugs for hairloss and maintain clean/healthy lives and then there’s these mf’s in the 90’s juiced to the gills with steroids and have better hair than all of us lol

in Satire 353 upvotes 2 years ago

The conversation is about the effects of steroids on hair loss. Some users believe that steroids can cause hair loss, while others argue that it depends on individual sensitivity to DHT. There is also discussion about the appearance of balding individuals who use steroids.

community End of Hair loss ( AGA ) ? Since they produce it in china probably we don’t need to wait other 10 yrs😁

in Treatment 261 upvotes 3 days ago

GT20029 is a new hair loss treatment in Phase 3 trials in China, using PROTAC technology to target androgen receptors, potentially with fewer side effects than finasteride and minoxidil. VDPHL01, a second-generation minoxidil, is also mentioned as potentially more effective.

community UPDATE: The theory that explains everything. Please help me make this big!

in Research/Science 445 upvotes 4 years ago

Hair loss theory involves 3alpha-hydroxysteroid reductase (3AHD) converting DHT to androstenol. Discussion explores potential treatments and encourages more research.

community If You Have DUPA, PLEASE READ THIS: Everyone Should Be Scalp Biopsied

nkrata in Research 830 upvotes 1 year ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

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Quantitative Analysis of the Anatomical Changes in the Scalp and Hair Follicles in Androgenetic Alopecia Using Magnetic Resonance Imaging

research Quantitative Analysis of the Anatomical Changes in the Scalp and Hair Follicles in Androgenetic Alopecia Using Magnetic Resonance Imaging

2 citations , June 2020 in “Skin Research and Technology”

MRI reveals thinner scalps and smaller hair follicles in people with androgenetic alopecia.