Protein loss quantification of abraded virgin and abraded bleached hair according to Bradford assay

This study demonstrated that the Bradford assay was a viable alternative to the Lowry test for quantifying hair damage during combing or brushing by measuring protein loss. The Bradford method, which involves the binding of Coomassie Brilliant Blue G-250 to keratin, was found to be rapid, sensitive, and less prone to interference from common hair care ingredients compared to the Lowry procedure. The study showed that virgin hair released significantly less protein (0.875 to 1.03 mg/g) than bleached hair (4.85 to 5.35 mg/g) during abrasive treatments, indicating greater damage in bleached hair. The results were reproducible, and the developed color remained stable for up to one hour.