Porous Hydrogel of Wool Hair Keratin as a Substrate for Cell Culture

    January 2016 in “ Frontiers in Bioengineering and Biotechnology ”
    Yuki Ozaki, Yusuke Takagi, Mori Hideki, Hara Masayuki
    TLDR A wool hair keratin hydrogel is promising for growing cells and tissue engineering.
    The study presented a novel method for creating a sponge-like porous hydrogel from wool hair keratin, which was shown to be a promising substrate for cell culture. The keratin was extracted and formed into a hydrogel with a highly porous structure and fast-swelling properties. It demonstrated high mechanical strength and flexibility, making it suitable for use as a scaffold in tissue engineering. The hydrogel supported the attachment and growth of various animal cells, including PC12 cells, HOS cells, and murine embryonic fibroblasts, as observed through fluorescent microscopy and scanning electron microscopy. Preliminary studies indicated potential applications in bone tissue engineering by culturing osteoblasts on the keratin hydrogel.
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