Peer Review of Establishment of an Immortalized Mouse Dermal Papilla Cell Strain with Optimized Culture Strategy

The document is a peer review of a study that aimed to optimize the isolation and culture methods of dermal papilla (DP) cells and establish an immortalized line. The reviewers highlighted several issues with the initial versions of the study. They pointed out that the reason for establishing an immortalized line was not clear and that the method of isolation appeared the same as that described in existing literature. They also questioned the choice of control media and the lack of detail about the number of times certain protocols were performed. The reviewers suggested that the study should include comparisons with non-immortalized DP cells and provide more information about the optimization of the media used for culturing DP cells. The authors were also advised to clarify certain terms and concepts used in the study. The final version of the study was accepted after the authors addressed these comments.