Peer Review of Establishment of an Immortalized Mouse Dermal Papilla Cell Strain with Optimized Culture Strategy
January 2018
TLDR Researchers improved mouse skin cell culture methods and created a similar immortal cell line, but need to clarify their methods and benefits.
In 2018, a study was conducted to optimize the isolation and culture methods of dermal papilla (DP) cells and establish an immortalized line. The researchers made improvements to the media used for culturing these cells, adding sodium pyruvate, non-essential amino acids, and FGF2. However, it was noted that FGF2 had little effect by itself. The study also attempted to establish an immortalized DP cell line, with iDP6 identified as the optimal induced line due to its similarity to primary DP cells. The reviewers suggested that the methods and results sections needed more detail, including the number of times experiments were performed and the inclusion of primary DP cells in the comparison. They also recommended clarifying the benefits of an immortalized line over a primary line and explaining how the new isolation method made DP isolation easier.
