Determination of Oxymatrine in Human Plasma by HPLC-MS

    January 2006
    Jin Ren
    TLDR A method was created to measure oxymatrine in human blood.
    A HPLC-MS method was developed to measure oxymatrine levels in human plasma, aiding in the pharmacokinetic study of its capsules. Finasteride served as the internal standard, and a C18 column was utilized with a specific mobile phase composition. The method employed electrospray ionization for detection, with m/z values of 265.2 for oxymatrine and 373.3 for finasteride. The calibration curve demonstrated linearity within the range of 2.5 to 500 ng/ml.
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