Protocol for Optimized Dissociation of Human Scalp Tissue for Hair Follicle Transcriptomics by scRNA-Seq

This protocol presents an optimized method for dissociating human scalp tissue to obtain various cell types for single-cell RNA sequencing (scRNA-seq), achieving approximately 200,000 cells with over 85% viability from a 25 mm² sample. It addresses common issues like intact tissue pieces and low cell yields by ensuring proper enzyme activity and careful handling. While the method is crucial for high-quality RNA data and applicable to other skin tissues, it may bias towards cell types easier to digest enzymatically. The protocol focuses on maintaining sample activity and precise sequencing preparation without introducing new reagents or datasets.