Optimization of Bear Oil Extraction Process and Hair Growth Activity

The study optimized the enzymatic extraction process of bear oil, achieving an extraction rate of 81.89%, significantly higher than the traditional method's 20-30%. Optimal conditions included pH 6.5-7, temperature 52-54°C, liquid-feed ratio 2:1, enzyme digestion time 4.5-5 hours, and enzyme addition of 5000-6000 U·g−1. GC-MS analysis revealed that bear oil extracted enzymatically had a higher content of unsaturated fatty acids compared to the traditional boiling method. Pharmacodynamic experiments on 50 KM mice showed that a hair regrowth gel containing bear oil significantly increased hair weight and follicle number, particularly in the high-dose group, indicating its potential for promoting hair growth.