Development of a New GC-MS/MS Method for the Determination of Metformin in Human Hair
June 2020
in “
Drug Testing and Analysis
”
metformin gas chromatography-tandem mass spectrometry GC-MS/MS human hair forensic purposes clinical purposes limit of detection limit of quantification linearity repeatability reproducibility diabetic patients drug incorporation hair concentration dark hair light hair monitoring therapy medical conditions LOD LOQ
TLDR A new method was created to measure metformin in hair, showing potential for monitoring treatment and medical investigations.
The document reports on a new gas chromatography-tandem mass spectrometry (GC-MS/MS) method developed to measure metformin levels in human hair, which could be significant for forensic and clinical purposes. The method showed a limit of detection (LOD) of 1 pg/mg and a limit of quantification (LOQ) of 100 pg/mg, with good linearity, repeatability, and reproducibility. The study analyzed hair from 9 diabetic patients on metformin, finding concentrations between 0.3 and 3.8 ng/mg, which indicates a low incorporation of the drug into hair. A potential correlation between the daily dose and hair concentration was observed in dark hair, but not in light hair. Despite the small sample size, the study offers preliminary insights for interpreting metformin levels in hair, which could be useful for monitoring therapy and investigating certain medical conditions.
