TLDR The method helps study hair follicle stem cells and calcium signals in mouse skin.
The document described a protocol for high-resolution whole mount staining and in vivo calcium imaging of hair follicle stem cells in mouse tail skin, using specific genetically modified mice. This method allowed researchers to study hair follicles and stem cells under various conditions, and it could be adapted to visualize calcium signaling in other cell types or analyze different cellular features using alternative reporter lines.
23 citations
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October 2021 in “Cell Stem Cell” Hair thinning causes stem cell loss through a process involving Piezo1, calcium, and TNF-α.
56 citations
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June 2015 in “Nature Protocols” Two-photon microscopy helps observe hair follicle stem cell behaviors in mice.
23 citations
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October 2021 in “Cell Stem Cell” Hair thinning causes stem cell loss through a process involving Piezo1, calcium, and TNF-α.
18 citations
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November 2020 in “Frontiers in Cell and Developmental Biology” Inflammation plays a key role in activating skin stem cells for hair growth and wound healing, but more research is needed to understand how it directs cell behavior.
36 citations
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February 2018 in “British Journal of Dermatology” Sweat glands and hair follicles are structurally connected within a specific layer of skin fat.
19 citations
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September 2019 in “EMBO molecular medicine” Blocking TSLP reduces skin inflammation and cell overgrowth in psoriasis.
30 citations
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November 2018 in “EMBO Reports” The Ovol2-Zeb1 circuit is crucial for skin healing and hair growth by guiding cell movement and growth.
