Identification and Profiling of MicroRNA Between Back and Belly Skin in Rex Rabbits (Oryctolagus Cuniculus)
June 2018
in “
World rabbit science
”
microRNA miRNA cell cycle regulation skin epithelium differentiation keratinocyte proliferation hair follicle development melanogenesis Hedgehog pathway Wnt pathway Osteoclast differentiation MAPK pathway quantitative reverse transcription polymerase chain reaction GO enrichment signalling pathway analysis qRT-PCR gene ontology enrichment signalling pathways
TLDR Different miRNAs in Rex rabbit skin affect cell processes and hair growth.
The study identified and profiled 211 differentially expressed microRNAs (miRNAs) between the back and belly skin of Rex rabbits, with 90 upregulated and 121 downregulated. These miRNAs were involved in crucial biological processes such as cell cycle regulation, skin epithelium differentiation, keratinocyte proliferation, hair follicle development, and melanogenesis. The research highlighted the roles of miRNA-regulated target genes in key signalling pathways, including Hedgehog, Wnt, Osteoclast differentiation, and MAPK pathways. Nine candidate miRNAs and 5 predicted target genes were verified using quantitative reverse transcription polymerase chain reaction, and a regulatory network was constructed through GO enrichment and signalling pathway analysis. Further research was recommended to validate these regulatory relationships.
