Analysis of Methyltestosterone and Testosterone Propionate in Dried Blood Spots After Transdermal Application

The study investigated the detection of 17α-methyltestosterone (MeT) and testosterone propionate (TP) in dried blood spots (DBS) after transdermal application of a cream-based formulation used for hair growth. Using a high throughput liquid chromatography–tandem mass spectrometry method, MeT was detectable up to 97 hours and identifiable up to 97 hours after application, while TP was detectable up to 97 hours and identifiable up to 49 hours. The study involved 5 male subjects and highlighted the potential for contamination from MeT and TP on the skin during sampling. The method provides direct confirmation of the presence of these drugs in DBS.