Analysis of Methyltestosterone and Testosterone Propionate in Dried Blood Spots After Transdermal Application

June 2025 in “ Drug Testing and Analysis ”

Asami Miyamoto, Masato Okano, Masanori Ota, Mitsuhiko Sato

methyltestosterone testosterone propionate transdermal application dried blood spots liquid chromatography–tandem mass spectrometry hair growth MeT TP DBS LC-MS/MS

TLDR The method effectively detects MeT and TP in dried blood spots after cream application.

This study investigated the detection of 17α‐methyltestosterone (MeT) and testosterone propionate (TP) in dried blood spots (DBS) after transdermal application of a cream-based formulation used for hair growth. Using a high throughput liquid chromatography–tandem mass spectrometry method, MeT and TP were successfully detected in DBS samples from 5 male subjects. MeT was detectable up to 97 hours after application, while TP was detectable up to 97 hours and identifiable up to 49 hours. The study highlights the potential for contamination from residual drugs on the skin during sampling, but confirms the method's effectiveness in detecting these substances in DBS.

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