Direct Quantitative Digital Autoradiography Of Testosterone Metabolites In The Pilosebaceous Unit: An Environmentally Advantageous Trace Radioactive Technology

The study developed a methodology for directly quantifying testosterone metabolites in the pilosebaceous unit using digital autoradiography, which allowed for the detection of signals as weak as 25 dpm and calibration curves in the range of 50–500 dpm. The technique demonstrated high sensitivity, reproducibility, and practicability, with coefficients of variation not exceeding 10% for biological measurements. This method provided a comprehensive analysis of testosterone metabolism into its various derivatives and offered an environmental advantage by minimizing the use of radioactive materials.