Influence of Melatonin on HaCaT Cell Proliferation Measured by Means of ATP Bioluminescence and Fluorometric Assays
November 2002
TLDR Melatonin affects cell growth differently based on its concentration.
The study investigated the effects of melatonin on the proliferation of HaCaT keratinocytes. It was found that high concentrations of melatonin (1 nM) decreased DNA synthesis, indicating cytotoxic effects. In contrast, lower concentrations (10 μM - 1 nM) increased DNA synthesis after 24 hours, suggesting a stimulatory effect on cell proliferation. The research also indicated that melatonin's impact on hair growth was concentration-dependent, with optimal stimulation observed at concentrations between 10 and 40 μmol, peaking at 30 μmol. The study aimed to identify the optimal melatonin doses for enhancing HaCaT cell proliferation.