MALDI Imaging Mass Spectrometry of Skin: Aspects of Sample Preparation

The document from November 1, 2017, reviews the application of Matrix-Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI IMS) in the analysis of skin samples. It emphasizes the importance of sample preparation, including steps such as specimen collection, preservation, tissue embedding, cryosectioning, washing, and matrix application, which need to be optimized for the analytes of interest due to the unique properties of skin. The technique is highlighted for its utility in studying skin pathophysiology, drug absorption, metabolism, wound healing, neoplasia, infection, and the distribution of lipids in various skin conditions. The document also discusses the challenges of quantitation in MALDI IMS and the potential of the technique in monitoring treatment efficacy for diseases like Fabry's disease and leprosy. It concludes that MALDI IMS is a valuable tool for understanding skin physiology and pathology and should be more broadly used by pathologists to study a wider range of skin conditions. No specific number of people or studies is mentioned for the strength of the study to be assessed.