LC-APCI+-MS/MS Method for the Analysis of Ten Hormones and Two Endocannabinoids in Plasma and Hair from Mice with Different Gut Microbiota

This study developed a new method using liquid chromatography-tandem mass spectrometry to measure multiple steroid hormones in hair, requiring significantly fewer strands than previous methods, with high accuracy and precision.

This study developed a method to measure local steroid hormone concentrations in hair, providing new insights into hormone dynamics associated with androgenetic alopecia.

In this study, researchers developed an LC-MS/MS method to accurately quantify cortisone and cortisol levels in human scalp hair, finding that concentrations decrease predictably along the hair shaft, which could assist in identifying abnormal glucocorticoid exposure.

The document concludes that using LC-MS/MS for measuring androgens is more accurate than older methods, but it needs careful validation and standardized references to be most effective.