Inhibition of TNF-Alpha Induced Collagen Degradation and Oxidative Damage by Centipeda Minima and Brevilin A in Human Dermal Fibroblasts

The study demonstrates that Centipeda minima (CMX) and its active compound brevilin A effectively protect normal human dermal fibroblasts (NHDFs) from TNF-α-induced oxidative stress and inflammation. They achieve this by reducing levels of reactive oxygen species (ROS), nitric oxide (NO), and prostaglandin E2 (PGE2), inhibiting matrix metalloproteinase-1 (MMP-1) expression, and restoring procollagen I α1 (COLIA1) levels, thereby preserving the extracellular matrix (ECM) and counteracting skin aging. Brevilin A specifically inhibits ERK phosphorylation in the MAPK signaling pathway, suggesting its potential in preventing ECM degradation and inflammation. These findings indicate that CMX and brevilin A are promising natural agents for protecting against skin aging and inflammation, with further in vivo studies needed to validate their efficacy and explore potential applications in dermatological formulations.