Investigation of In Vitro and In Vivo Skin Permeability Using Laser Scanning Microscopy

The thesis investigated skin permeability using advanced optical imaging techniques, focusing on the penetration of molecules and nanoparticles. It found that nanoparticles, such as zinc oxide (ZnO-nano), generally remained in the stratum corneum and accumulated in skin folds and hair follicles, except when chemical enhancers were used. This suggested that sunscreen-based nanoparticles were unlikely to pose safety concerns. The study also explored the permeation pathways of low-molecular weight drugs using Rhodamine B, determining its diffusion coefficient, which aligned with known values. The research highlighted the potential for non-invasive imaging of nanoparticle penetration in skin care applications.