In Vitro Isolation and Cultivation of Human Axillary Dermal Papilla Cells

July 2018 in “ Chinese Journal of Dermatology ”

Chunling Huang, Nanlan Yu, Ya Xiong, Dongmei Zhang

dermal papilla cells enzyme digestion micro-dissection

TLDR The two-step enzyme digestion method is the best for isolating axillary dermal papilla cells.

The study aimed to find an efficient method for isolating and cultivating human axillary dermal papilla cells. Researchers compared three methods: two-step enzyme digestion, one-step digestion, and micro-dissection. The two-step enzyme digestion method proved to be the most effective, with a simpler procedure, over 30% separation rate, and 96% adhesion rate after 1 week. It also reduced cell emigration duration by 3-4 days and had a lower contamination rate compared to the micro-dissection method. Although it had a longer total operation duration, the actual operation time was shorter. The study concluded that the two-step enzyme digestion method was a simple, efficient, and rapid technique for isolating axillary dermal papilla cells using minimal specimens.

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