In Vitro Isolation and Cultivation of Human Axillary Dermal Papilla Cells

The study compared three methods for isolating human axillary dermal papilla cells: a modified two-step enzyme digestion method, a one-step enzyme digestion method, and a microdissection method. The modified two-step enzyme digestion method was found to be simpler and more efficient, achieving over 30% isolation efficiency and a 96% adhesion rate after 1 week. It also reduced cell migration time by 3-4 days compared to the other methods. Although the total time required was longer, the actual operation time was shorter, and the contamination rate was lower than with the microdissection method. The cultured cells initially grew in an aggregated manner, transitioning to non-aggregated growth after six generations. The study concluded that the modified two-step enzyme digestion method was a simple, efficient, and rapid technique for isolating axillary dermal papilla cells from small samples.