Influence of Conditioned Media from Glial Cell Cultures on Uterine Contractility in Rats of Different Ages

The study examined the impact of conditioned media (CM) from glial cell cultures on uterine contractility in rats of different ages, specifically 6-month-old (reproductive age) and 14-month-old (late reproductive age) rats. It was found that uterine contractions were reduced in older rats, but administration of CM restored these contractions and increased myometrium area, myocyte density, and smooth muscle actin expression. The effects were consistent whether the CM was from intact or cryopreserved cultures, indicating that cryopreservation maintained the CM's biological efficacy, suggesting its potential use in reproductive health interventions.