Influence of Conditioned Media from Glial Cell Cultures on Uterine Contractility in Rats of Different Ages

The study investigated the effects of conditioned media (CM) from glial cell cultures on uterine contractility in rats of different reproductive ages. It was found that in late reproductive age (LRA) rats, spontaneous, oxytocin (OT)-, and potassium chloride (KCl)-induced uterine contractions were reduced compared to younger rats. However, administration of CM from both intact and cryopreserved cultures normalized these contraction parameters in LRA rats. This improvement was associated with increased myometrium area, myocyte density, and smooth muscle actin expression. The study concluded that CM from glial cell cultures, whether intact or cryopreserved, enhanced uterine contractility in LRA rats, supporting the use of cryopreservation for maintaining the biological efficacy of CM.