Regulatory Network of Ginsenoside Biosynthesis Under Ro Stress in the Hairy Roots of Panax Ginseng Revealed by RNA Sequencing
October 2022
in “
Frontiers in bioengineering and biotechnology
”
TLDR Ro stress hindered ginseng root growth and ginsenoside production, but increased certain hormones and affected gene regulation related to plant growth and stress responses.
The study explored the impact of ginsenoside Ro stress on ginsenoside biosynthesis in Panax ginseng roots. It was found that 0.5 mg/L of Ro significantly inhibited root growth by 82.41% and reduced the accumulation of ginsenosides, except for Rg3. The Ro stress also altered the accumulation of endogenous hormones, promoting IAA and SA by 10.0% and 9.6% respectively, while reducing ABA and JA. RNA sequencing identified 74 differentially expressed miRNAs, with 22 upregulated and 52 downregulated. These miRNAs targeted genes mainly involved in auxin-activated signaling pathway, multicellular organism development, cell differentiation, and meristem initiation. The findings provide insights into the genetic engineering of plant stress responses and potential strategies for continuous ginseng production.
