Regulatory Network of Ginsenoside Biosynthesis Under Ro Stress in the Hairy Roots of Panax Ginseng Revealed by RNA Sequencing
October 2022
in “
Frontiers in bioengineering and biotechnology
”
Studysummary Ro stress hindered ginseng root growth and ginsenoside production, but increased certain hormones and affected gene regulation related to plant growth and stress responses.
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The study explored the impact of ginsenoside Ro stress on ginsenoside biosynthesis in Panax ginseng roots. It was found that 0.5 mg/L of Ro significantly inhibited root growth by 82.41% and reduced the accumulation of ginsenosides, except for Rg3. The Ro stress also altered the accumulation of endogenous hormones, promoting IAA and SA by 10.0% and 9.6% respectively, while reducing ABA and JA. RNA sequencing identified 74 differentially expressed miRNAs, with 22 upregulated and 52 downregulated. These miRNAs targeted genes mainly involved in auxin-activated signaling pathway, multicellular organism development, cell differentiation, and meristem initiation. The findings provide insights into the genetic engineering of plant stress responses and potential strategies for continuous ginseng production.
