Differential Gene Expression Screening Between Black and Gray Hairs Using Subtractive Library and DNA Chip

The study investigated differential gene expression between gray and black hairs using advanced molecular techniques, including RNA amplification, representational difference analysis, and microarray analysis. It found that genes related to pigment production, such as Pmel17, MART-1, and tyrosinase, were more abundantly expressed in black hairs. Additionally, genes like selenium-binding protein and spastin protein were up-regulated in black hairs. Conversely, gray hairs showed higher expression of genes associated with keratin, trichohyalin, and transmembrane glycoproteins, with a notable expression of ch 17, hRPK.142_H_19.