Effect of Gamma-Ray Exposure on the Genome-Editing Efficiency of Improved Genome-Editing via Oviductal Nucleic Acids Delivery (i-GONAD)

January 2025 in “ EXPERIMENTAL ANIMALS ”

Anarkhuu Bold-Erdene, Kento Miura, Norimasa Yamasaki, Shuka Miura, Sawako Ogata, Osamu Kaminuma, Takashi Yamamoto, Osamu Kaminuma

gamma-ray exposure genome-editing i-GONAD Hr gene hair loss sgRNA mosaic mutations indel mutations hairless genome editing small guide RNA

TLDR Gamma-ray exposure improves genome editing efficiency in mice using the i-GONAD method.

This study examined the effect of gamma-ray exposure on the genome-editing efficiency of the i-GONAD method in pregnant mice targeting the Hr gene, which is associated with hair loss. Gamma-ray exposure on the day of fertilization (Day 0) and the day after (Day 1) was found to enhance genome editing efficiency in a dose-dependent manner, despite reducing natural delivery rates and litter sizes at doses of 0.3 Gy or higher. While the proportions of hairless offspring varied with different sgRNAs, the overall mutation rates, including hairless, mosaic, and indel mutations, remained consistent. The findings suggest that gamma-ray exposure can improve genome editing efficiency in the i-GONAD method, aiding in the development of diverse experimental animal models.

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