Genosomes: An Introspection Into Transfection, Future Perspectives and Applications
September 2025
in “
Advanced Pharmaceutical Bulletin
”
Studysummary This review highlights the promise of non-viral gene delivery systems, particularly genosomes, over traditional viral vectors due to enhanced safety and efficiency in therapeutic nucleic acid delivery for genetic diseases like cystic fibrosis and malignancies.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
Genosomes, which are cationic lipid-based nanocarriers, have emerged as promising non-viral gene delivery systems due to their enhanced biosafety and lower immunogenic potential compared to traditional viral vectors. These systems efficiently condense nucleic acids, protect them from enzymatic degradation, and improve cellular uptake and endosomal escape. Advances such as PEGylation and stimuli-responsive formulations have further enhanced their transfection efficiency and tissue-specific delivery. Clinical applications, including RNA-lipoplexes in cancer immunotherapy, demonstrate their therapeutic potential. This review critically examines genosome design, formulation, intracellular trafficking, clinical applications, and future prospects in gene therapy.
