A Fluorescence-Based Lymphocyte Assay Suitable for High-Throughput Screening of Small Molecules
March 2017
in “
Journal of Visualized Experiments
”
Studysummary This study introduced a novel in vitro murine assay using transgenic mice to identify immunomodulatory compounds, which identified 160 potential hits from 4,398 compounds screened.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
The study introduced a novel fluorescence-based assay using lymphocytes from a transgenic mouse, designed for high-throughput screening (HTS) of small molecules that can modulate lymphocyte activation. This assay utilized T or B cells from a transgenic mouse where GFP expression is driven by the Nur77 promoter upon receptor stimulation, allowing the assessment of cellular activation through GFP intensity. A primary screening of 4,398 compounds identified 160 potential hits with immunomodulatory activities, demonstrating the assay's utility in drug discovery programs targeting large chemical libraries.
