A HPLC-Based Chloramphenicol Acetyltransferase Assay for Assessing Hair Growth: Comparison of the Sensitivity of UV and Fluorescence Detection

January 1993 in “ Clinical Chemistry and Laboratory Medicine (CCLM) ”

Daniel J. Waldon, Marc F. Kubicek, George Johnson, Ann Buhl

TLDR The new method is 1000 times more sensitive for measuring hair growth.

The study developed a highly sensitive chloramphenicol acetyltransferase assay using HPLC-fluorescence detection to measure hair growth in transgenic mice expressing the gene under a keratin promoter. This method achieved a sensitivity of less than 1 x 10(-13) mol, which was 1000 times greater than HPLC-UV detection, allowing for the analysis of enzyme activity in a single follicle and processing over 100 samples per day. The fluorescent assay was suggested to be applicable to various cell or tissue studies.

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research A HPLC-Based Chloramphenicol Acetyltransferase Assay for Assessing Hair Growth: Comparison of the Sensitivity of UV and Fluorescence Detection

4 citations , January 1993 in “Clinical Chemistry and Laboratory Medicine (CCLM)”

The new method is 1000 times more sensitive for measuring hair growth.