A HPLC-Based Chloramphenicol Acetyltransferase Assay for Assessing Hair Growth: Comparison of the Sensitivity of UV and Fluorescence Detection

January 1993 in “ Clinical Chemistry and Laboratory Medicine (CCLM) ”

Daniel J. Waldon, Marc F. Kubicek, George Johnson, Ann Buhl

TLDR The new method is 1000 times more sensitive for measuring hair growth.

The study developed a highly sensitive HPLC-based chloramphenicol acetyltransferase assay to assess hair growth using transgenic mice. By employing a fluorescent substrate, the assay achieved a sensitivity of less than 1 x 10^-13 mol, which was 1,000 times greater than the sensitivity of HPLC-UV detection. This enhanced sensitivity allowed for the measurement of enzyme activity in single hair follicles and facilitated the analysis of over 100 samples per day. The fluorescent assay was proposed to be applicable to various cell or tissue studies, providing a significant improvement over previous methods for detecting chloramphenicol acetyltransferase activity in hair growth research.

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research A HPLC-Based Chloramphenicol Acetyltransferase Assay for Assessing Hair Growth: Comparison of the Sensitivity of UV and Fluorescence Detection

4 citations , January 1993 in “Clinical Chemistry and Laboratory Medicine (CCLM)”

The new method is 1000 times more sensitive for measuring hair growth.