Determination of Finasteride in Healthy Volunteers by Optimized Liquid Chromatography-Tandem Spectrometry

January 2010 in “ World Clinical Drugs ”

GE Qing-hua

finasteride liquid chromatography-tandem spectrometry pharmacokinetics liquid-liquid extraction C18 column Propecia LC-MS/MS

TLDR The method accurately measures finasteride levels in the body.

The study established an optimized liquid chromatography-tandem spectrometry (LC-MS/MS) method for determining finasteride in 20 healthy volunteers, evaluating its pharmacokinetics. Plasma samples underwent liquid-liquid extraction, and chromatographic separation was achieved using a C18 column with a specific mobile phase. The method demonstrated excellent linearity over a concentration range of 0.5-75 ng/mL, with intra- and inter-assay precision within 7.0% and assay accuracy between 86.44% and 115.5%. The optimized LC-MS/MS method was found to be sensitive and accurate, successfully applied in the pharmacokinetic study of finasteride tablets.

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