Determination of Finasteride in Healthy Volunteers by Optimized Liquid Chromatography-Tandem Spectrometry
January 2010
in “
World Clinical Drugs
”
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4 / 4 resultsresearch A Rapid, Simple, Liquid Chromatographic-Electrospray Ionization, Ion Trap Mass Spectrometry Method for the Determination of Finasteride in Human Plasma and Its Application to Pharmacokinetic Study
research Determination of Finasteride in Human Serum by LC-MS/MS
research A Rapid, Simple, Specific Liquid Chromatographic–Electrospray Mass Spectrometry Method for the Determination of Finasteride in Human Plasma and Its Application to Pharmacokinetic Study
Method measures finasteride in plasma, suitable for pharmacokinetic studies.
research Determination of Finasteride in Human Plasma by HPLC-MS
The study developed a high-performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) method to determine finasteride levels in human plasma. The method used a mobile phase of ammonium acetate/water and methanol, with plasma samples extracted using ethyl acetate and separated by a C_18 column. The results showed a linear standard curve for finasteride concentration ranging from 0.491 to 98.2 μg·L^-1, with a high correlation coefficient (r=0.9999). The method demonstrated a relative recovery of 90%-110%, with intra-day and inter-day relative standard deviations (RSD) under 5%. The quantitation limit was 0.49 μg·L^-1, and the extraction recovery exceeded 90%. The study concluded that this method was stable and reliable for determining finasteride in human plasma and suitable for pharmacokinetic studies.