Exosomes Facilitate mRNA and siRNA Delivery Using Cationic Liposomes 2X3-DOPE to Rat Heart Mesenchymal Cells In Vitro

    June 2025 in “ Medical academic journal ”
    Olesya Dovbysh, Vera V. Vysochinskaya, Nina V. Gavrilova, Pavel M. Docshin, Ekaterina Nikitina, Aleksandr S. Klochev, Е. А. Елпаева, Olga A. Dobrovolskaya, Elena V. Shmendel, М. А. Маслов, Yana Zabrodskaya
    TLDR Modified liposomes with exosomes effectively deliver RNA to stem cells.
    The study investigates the delivery of mRNA and siRNA to rat heart mesenchymal stem cells using cationic liposomes 2X3-DOPE and hybrid nanoparticles incorporating exosomes. The results show that while 2X3-DOPE alone is ineffective for mRNA delivery, hybrid nanoparticles with exosomes achieve up to 40% transfection efficiency. For siRNA, 2X3-DOPE modified with DSPE-PEG2000 (0.62 mol%) achieves up to 90% transfection efficiency, and when combined with exosomes, results in 100% transfection with a significant increase in siRNA uptake. The study concludes that these modified liposomes, especially when combined with exosomes, are promising for RNA delivery to mesenchymal stem cells.
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