Novel Method for the Estimation of Minoxidil and Aminexil in Marketed Formulation
February 2025
in “
International Journal For Multidisciplinary Research
”
Studysummary This study developed and validated a simple, accurate, and sensitive HPTLC method for estimating minoxidil and aminexil in topical solutions, demonstrating effective separation and linear measurement of these compounds with %RSD under 2% and high regression coefficients.
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The study presents a validated HPTLC method for estimating Minoxidil and Aminexil in topical solutions, using silica gel 60 F254 pre-coated TLC aluminium plates and a methanol: water (5:5) solvent system. Densitometric analysis at 254 nm revealed Rf values of 0.88 for Aminexil and 0.69 for Minoxidil, with linearity between 2-10 μg/spot for Minoxidil and 6-14 μg/spot for Aminexil. The method demonstrated high precision and accuracy, with %RSD below 2% and regression coefficients of 0.9983 for Minoxidil and 0.9993 for Aminexil, meeting ICH criteria.
