Application of Complex Filling Material Consisting of Autologous Skin Fibroblast and Hair Keratin in Medical Cosmetology

The study investigated the use of a complex filling material made from autologous skin fibroblasts (Fbs) and hair keratin (HK) for reducing facial wrinkles. Fbs were cultured with HK particles and injected into the facial wrinkles of 10 volunteers. The optimal ratio of HK to Fbs was found to be 1:2, which maximized fibroblast survival, proliferation, and collagen I production. Four weeks post-injection, the treated skin was soft and compliant without stiffness, and the volunteers' appearances improved significantly. This demonstrated the material's potential for clinical application in medical cosmetology.