Synchronously Developing Collet Hairs in Arabidopsis Thaliana Provide an Easily Accessible System for Studying Nuclear Movement and Endoreduplication

The study investigated the growth of collet hairs in Arabidopsis thaliana seedlings, focusing on the associated nuclear movement and endoreduplication. Researchers used fluorescent probes, time-lapse imaging, and flow cytometry to observe these processes in a double-transgenic Arabidopsis hybrid line. They found that while the nucleus tends to be near the cell tip during hair growth, this positioning is not crucial for the hair's tip-directed growth. The nuclear DNA content was observed to increase from 4C to 16C during hair development. After growth stopped, the nuclei moved to the base of the hairs and their movement became less synchronized. The study also noted that nuclear movement was affected by the presence of larger prevacuolar vesicles. The collet hairs of Arabidopsis were presented as a suitable model for studying nuclear movement and endoreduplication due to their natural synchronization and accessibility.