Molecular Cloning of Two Novel Types of Peptidylarginine Deiminase cDNAs from Retinoic Acid-Treated Culture of a Newborn Rat Keratinocyte Cell Line
August 1998
in “
FEBS Letters
”
TLDR Two new enzymes, PAD-R11 and PAD-R4, were cloned and showed activity, with PAD-R11 resembling epidermal enzymes.
The study investigated the molecular cloning of two novel peptidylarginine deiminase (PAD) cDNAs, PAD-R11 and PAD-R4, from retinoic acid-treated newborn rat keratinocyte cells. These novel PADs showed significant sequence similarity to known PAD types II and III and demonstrated specific enzymatic activities, suggesting their involvement in epidermal differentiation. Northern blot analysis confirmed that retinoic acid induced the expression of these PAD mRNAs, indicating their potential roles in the terminal stages of epidermal differentiation. The study also identified PAD-R11 as encoding PAD type I and PAD-R4 as tentatively classified as type IV, with their expression modulated by retinoic acid, highlighting the need for further research on PAD expression and function in protein deimination during degenerative processes.
