A Partially Automated Method for DNA Extraction from Marmoset Hair Follicles to Avoid Blood Chimerism

    September 2025 in “ Frontiers in Genetics
    Alexandra M. Stendahl, Qiangge Zhang, Ana C. Lima, Curtis J. Mello, James Nemesh, S. Peterson, Jenna N. Castro, Fritzie T. Celino‐Brady, Karina Ray, Xian Gao, Yuanyuan Hou, Chenjie Shen, Katinka A. Vigh‐Conrad, Fenna M. Krienen, Guoping Feng, Steven A. McCarroll, Donald F. Conrad, Ricardo C.H. del Rosario
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    Studysummary This study developed a non-invasive, partially automated protocol for extracting high-quality DNA from hair follicles of marmosets, significantly reducing chimerism rates compared to blood, and proving reliable for whole genome sequencing in low-input DNA scenarios.
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