Antioxidant Activity Test Combination of Cantigi Leaf Extract (Vaccinium Varingiaefolium) and Nail Henna Leaf Extract (Lawsonia Inermis Linn)

The study evaluated the antioxidant activity of cantigi leaf extract (CLE) and nail henna leaf extract (NHLE) using the DPPH method. CLE alone showed moderate antioxidant activity with an IC50 value of 119.23 µg/mL, while NHLE had very strong activity with an IC50 of 38.38 µg/mL. Combining these extracts in different ratios resulted in a synergistic effect, significantly enhancing antioxidant activity. The optimal combination was a 1:2 ratio of CLE to NHLE, achieving an IC50 value of 29.85 µg/mL, indicating a very strong antioxidant potential.