Analytical Methods on the Determination of Active Ingredients for Hair Nourisher Products
January 2010
TLDR The new method accurately measures active ingredients in hair nourishment products.
The study aimed to develop and validate simultaneous analytical methods for determining active ingredients in hair nourisher products, which are used to prevent hair loss and classified as quasi-drugs in Korea. The ingredients analyzed included nicotinamide, tocopherol, salicylic acid, dexpanthenol, and benzyl nicotinate using HPLC with a C18 column and acetonitrile/phosphate buffer as the mobile phase. Detection was performed at UV wavelengths of 220, 270, and 300 nm. The calibration curve showed linearity in the range of 12.5 to 800 μg/mL, with recoveries of 97.3 to 103.5% (RSD 0.9 to 2.8%) in liquid matrix and 101.9 to 115.9% (RSD 0.7 to 7.7%) in shampoo matrix. The method was successfully applied to commercial hair nourisher products, all of which met the criteria, suggesting the method's utility for quality control and development of hair nourisher products.
