All-In-One Polysaccharide Hydrogel With Resistant Vascular Burst Pressure And Cooperative Wound Microenvironment Regulation For Fatal Arterial Hemorrhage And Diabetic Wound Healing

The study presents the development of all-in-one beta-glucan (BG) hybrid hydrogels reinforced with laponite nanoclay, designed to address fatal arterial hemorrhage and diabetic wound healing. These hydrogels exhibit tunable tissue adhesion, high vascular burst pressure, and cooperative wound microenvironment regulation. They feature a honeycomb-like porous structure, with pore sizes ranging from 7-19 μm, tissue adhesion strength of 18-46 kPa, and vascular burst pressure of 58-174 mmHg. In rat liver and femoral artery models, the hydrogels demonstrated superior hemostasis, scavenged reactive oxygen species, and transformed macrophages from proinflammatory M1 to prohealing M2. A single treatment with NO-releasing BG hybrid hydrogels led to complete closure of diabetic wounds within 14 days, promoting epithelization, dermis growth, and normal vascularization, along with optimal collagen deposition and hair follicle regeneration. This innovative approach opens new avenues for designing polysaccharide hydrogels for effective hemostasis and diabetic wound dressings.