High-Performance Liquid Chromatographic Method for the Determination of Finasteride in Human Plasma at Therapeutic Doses

May 1991 in “ Journal of Chromatography B: Biomedical Sciences and Applications ”

M.L Constanzer, Bogdan J. Matuszewski, W.F. Bayne

finasteride high-performance liquid chromatography HPLC human plasma Propecia

Studysummary This study developed and validated an HPLC method for detecting a novel 5α-reductase inhibitor in human plasma, supporting its pharmacokinetic evaluation at low doses.

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This document describes a validated high-performance liquid chromatographic method for detecting finasteride in human plasma at therapeutic doses. The method was found to be reliable and efficient for quantifying finasteride in human plasma at doses down to 5 mg. The authors also discuss the challenges of detecting finasteride using HPLC due to its low UV absorbance and lack of a functional group for derivatization. Overall, the method was found to be practical and highly reliable.

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research Effects of Finasteride (MK-906), a 5α-Reductase Inhibitor, on Circulating Androgens in Male Volunteers

147 citations , April 1990 in “The Journal of Clinical Endocrinology and Metabolism”

This study found that finasteride significantly suppresses serum dihydrotestosterone levels at all tested doses in normal male volunteers without significant adverse effects.

research Species Differences in Prostatic Steroid 5α-Reductases of Rat, Dog, and Human

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This study found significant species differences in the enzyme activity and inhibitor affinities of prostatic 5α-reductases from rats, dogs, and humans, with variable potencies for different 3-oxo-4-azasteroid inhibitors across species.