5-Aminolevulinic Acid Improves Chicken Sperm Motility

The study found that 5-Aminolevulinic acid (5-ALA) significantly improved chicken sperm motility. The optimal concentration was found to be 0.1 mM, beyond which the motility started to decrease. The addition of 0.05 mM 5-ALA increased various aspects of sperm motility, including progressive motility, linearity, average path velocity, curvilinear velocity, straight-line velocity, and the wobble. It also increased sperm mitochondrial membrane depolarization, which is thought to utilize ATP for enhancing sperm movement. However, 5-ALA treatment decreased sperm ATP levels. The study suggests that 5-ALA could potentially be used to enhance sperm motility in chickens, but the concentration needs to be carefully controlled.