3D Imaging of Cleared Ex Vivo Normal Human Skin, Skin Appendages, and Psoriasiform Skin Lesion Using Light-Sheet Microscopy

The study demonstrated that light-sheet fluorescence microscopy (LSFM), when combined with optical clearing using a benzyl alcohol and benzyl benzoate solution, can effectively create 3D images of human skin biopsies. The researchers were able to make formalin-fixed human skin biopsies transparent and used LSFM for detailed imaging of skin structures, including normal skin, skin appendages, and a psoriasiform skin lesion. The imaging revealed epidermal hyperplasia in the pathological sample and allowed for 3D reconstruction to compare epidermal layer thickness and volume between normal and psoriatic skin. The study concluded that these techniques provide valuable new applications for high-resolution 3D visualization and quantification in dermatological research.