Three-Dimensional Culture of Dermal Papilla Cells Improves Their In Vitro Angiogenic Activity
November 2022
in “
Journal of Investigative Dermatology
”
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Studysummary This study observed that three-dimensional cultures of dermal papilla cells enhanced endothelial cell migration and angiogenesis in vitro, which may improve vascularization in tissue-engineered skin constructs. Our plain-language summary of this paper — not a Tressless recommendation.
The study demonstrates that three-dimensional culture of dermal papilla cells (DPCs) enhances their angiogenic capabilities in vitro. Culturing DPCs as spheres increases the expression of angiogenic genes such as VEGF, angiogenin, and FGF, which are crucial for vascularization. The researchers used two angiogenic study models to compare the ability of monolayer and sphere cultures of DPC to promote endothelial cell migration and neovessel development. The results showed increased HUVEC migration and an increase in the total number of segments, joints, and networks, which are characteristic hallmarks of angiogenesis, when HUVEC were seeded in spheres conditioned media. This suggests that the use of DPC spheres in skin substitutes may enhance the vascularization of grafts, thereby improving wound healing and graft take.
