Bone-Forming Cells with Pronounced Spread into the Third Dimension in Polymer Scaffolds Fabricated by Two-Photon Polymerization
December 2016
in “
Journal of Biomedical Materials Research Part A
”
mesenchymal progenitor cells human hair roots polymer scaffolds two-photon polymerization Ti-sapphire femtosecond laser osteogenic lineage hydrophilic 3D scaffolds calcium-binding proteins actin cytoskeleton extracellular matrix stem cells hair root cells 3D scaffolds laser polymerization bone cells calcium proteins cell matrix
TLDR Bone-forming cells grow well in 3D polymer scaffolds with 35 µm pores.
The study aimed to stimulate bone-forming cells to create 3D networks of mineralized proteins by using mesenchymal progenitor cells from human hair roots seeded onto polymer scaffolds. These scaffolds were fabricated using two-photon polymerization with a Ti-sapphire femtosecond laser. The cells, differentiated into an osteogenic lineage, were seeded onto hydrophilic 3D scaffolds, leading to high production of calcium-binding proteins and increased actin cytoskeleton activation. The optimal results were achieved with quadratic pore sizes of 35 µm, where the pores were nearly filled with cells and extracellular matrix material.
