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Glossary Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

advanced technique for separating and identifying compounds in samples

Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS/MS) is an advanced analytical technique used to separate, identify, and quantify compounds in a sample. UPLC enhances the resolution and speed of liquid chromatography, while MS/MS provides highly sensitive and specific detection by breaking down molecules into fragments and analyzing their mass. This method is widely used in fields like pharmacology, biochemistry, and environmental science for precise and accurate analysis of complex mixtures.

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Ultra-Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Ketoconazole in Anti-Dandruff Shampoo

research Ultra-Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Ketoconazole in Anti-Dandruff Shampoo

1 citations , February 2014 in “Analytical Letters”

This study developed a fast and sensitive ultra-high performance liquid chromatography method to quantify ketoconazole in antidandruff emulsions, showing comparable results to labeled concentrations.

Simultaneous Determination of Androgens and Prostaglandins in Human Urine Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

research Simultaneous determination of androgens and prostaglandins in human urine using ultra-high-performance liquid chromatography–tandem mass spectrometry

10 citations , January 2019 in “Journal of Chromatography B”

This study used a newly validated method to find that one-year finasteride treatment resulted in no significant differences in urinary androgen and prostaglandin levels between male pattern baldness patients and normal controls.

research Determination of 13 janus kinase inhibitors in anti-alopecia cosmetics by ultra-high performance liquid chromatography-tandem triple quadrupole composite linear ion trap mass spectrometry

September 2025 in “Chinese Journal of Chromatography”

This study developed a method using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MRM-IDA-EPI) for the rapid and sensitive detection of 13 JAK inhibitors in anti-alopecia cosmetics, showing excellent separation and accurate quantification with high recovery rates in tested matrices.

research Simultaneous determination of androgens and prostaglandins in human urine using ultra-high-performance liquid chromatography–tandem mass spectrometry

January 2019

This study found no significant difference in urinary androgen and prostaglandin levels between male pattern baldness patients treated with finasteride for one year and normal controls.

research Studies on the persistence of estradiol benzoate and nortestosterone decanoate in hair of cattle following treatment with growth promoters, determined by ultra-high-performance liquid chromatography–tandem mass spectrometry

18 citations , April 2009 in “Journal of Chromatography A”

Detecting steroid esters in cattle hair is inconsistent and influenced by sampling factors.

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