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This study examines various methodological factors affecting hair cortisol concentration analysis, finding inconsistent results on the impact of factors like resuspension solution, hair weight, extraction duration, and hair-cutting method.

This study validated a method for measuring cortisol levels in hair, which may serve as a biomarker for chronic stress in both humans and various animals.

This study developed and validated a method for extracting cortisol from human and animal hair, suggesting its potential as a biomarker for assessing chronic stress exposure.

This study observed that hair cortisol concentrations in primates are influenced by factors such as age, sex, hair growth rates, and sampling methods, which must be accounted for in research designs.

This study suggests that collecting hair samples from all scalp regions may be a viable alternative for measuring cortisol in individuals with Afro-textured or extremely short hair, aligning results with traditional methods.

This study developed a validated method using UPLC-MS/MS to analyze 37 hormones in human hair, enabling broader hormonal profiling for identifying endocrine disorders.

This study found that while pulverization yielded slightly higher hair cortisol extraction, the significant interindividual differences observed suggest that any of the methods could be used effectively.

This study found that while the reproducibility of hair cortisol measurements in captive chimpanzees was high, the fineness of homogenized hair, sample weight, and extraction time influenced absolute cortisol concentrations.

This study found that sampling hair for cortisol analysis in mother-toddler dyads living in low-income U.S. homes is feasible, with 66% of approached dyads participating.

This study found that a hair growth rate of 0.75 cm/month better matches retrospective cortisol timelines and identified factors influencing hair cortisol content in children and adolescents.

This study developed a new method using liquid chromatography-tandem mass spectrometry to measure multiple steroid hormones in hair, requiring significantly fewer strands than previous methods, with high accuracy and precision.

This study found that LC-MS/MS assays provided more reliable and precise steroid measurements in human serum compared to traditional immunoassays, particularly at lower concentrations.

This study used a novel extraction method to detect and measure mineralocorticoids and glucocorticoids in hair follicles, concluding these steroids may serve as effective biomarkers for diagnosing conditions related to steroid excess or deficiency, such as Cushing’s syndrome and congenital adrenal hyperplasia.

Proximal hair cortisol is a reliable tool for diagnosing Cushing's Syndrome.

This study suggests that cortisol measured in human hair can effectively reflect increased production for up to six months, but its retrospective accuracy may be limited due to rapid decline in levels.

This study found that cortisol levels, a biomarker of chronic stress, significantly increased in the post-COVID-19 lockdown period compared to pre-lockdown and during-lockdown periods, especially among females and young adults, reflecting sustained psychological stress from the pandemic.

In this study, university students given open-label placebo treatment reported reduced psychological distress and lower hair cortisol concentrations during stressful exams, suggesting that these placebos may help manage the physiological and psychological impacts of prolonged stress.

This study observed that in privately owned, intact cats, male cats exhibited significantly higher long-term hair cortisol concentrations compared to females, while environmental and behavioral factors did not significantly impact cortisol levels, suggesting individual traits may influence stress markers more than external factors.

This study observed that hair loss in captive rhesus macaques varies with season, sex, and housing conditions, and is significantly correlated with HPA axis activity as indicated by faecal cortisol metabolites.

Darker hair may lead to higher cortisol readings, suggesting a need to adjust for hair color in studies.

In this study, scalp hair sweating was a significant predictor of hair cortisol levels in healthy adults, while BMI and perceived stress, although associated, did not significantly predict cortisol levels.

This article reviews the use of hair cortisol concentration as a biomarker to assess long-term stress in both animals and humans, but it reports no new experimental results.

This study suggests that measuring cortisol levels in hair may serve as a useful biomarker for assessing mood in individuals with bipolar disorder over recent months.

In this study, washing rhesus monkey hair with water or shampoo significantly reduced cortisol levels, suggesting potential underestimation of chronic stress hormone deposition in hair due to washing.

This study described a new assay for measuring cortisol in hair and found it may serve as a useful biomarker for long-term cortisol exposure, correlating with 24-hour urine cortisol levels in non-obese subjects.

In this study, researchers found that tritiated cortisol was incorporated into the hair of rhesus monkeys primarily as cortisol and cortisone, as well as other metabolites, after systemic administration.

In this study, researchers developed an LC-MS/MS method to accurately quantify cortisone and cortisol levels in human scalp hair, finding that concentrations decrease predictably along the hair shaft, which could assist in identifying abnormal glucocorticoid exposure.

This study observed that drug-naïve first-episode psychosis patients had higher hair cortisol concentrations than healthy controls, and these concentrations correlated with the severity of psychopathology.

This study found that men with androgenic alopecia had significantly higher levels of certain urinary steroid metabolites, suggesting increased 5 alpha-reductase activity and possible mild hyperadrenal activity.

Cortisone and cortisol levels in pregnant women’s hair increase as labor approaches, indicating higher stress.