13 citations
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September 2013 in “Steroids” This study found that a commonly-used DHT enzyme immunoassay shows significant cross-reactivity with testosterone, leading to inaccurate DHT measurements compared to a gold-standard LC–MS/MS method.
9 citations
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November 2022 in “International Journal of Molecular Sciences” This study found that LC-MS/MS assays provided more reliable and precise steroid measurements in human serum compared to traditional immunoassays, particularly at lower concentrations.
3 citations
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February 2025 in “Endocrine” This study emphasizes the necessity of confirming elevated testosterone levels detected by immunoassay with liquid chromatography-tandem mass spectroscopy before pursuing more complex diagnostic evaluations.
June 2025 in “JCEM Case Reports” In this case study, authors reported that a 43-year-old female patient initially showed persistently high testosterone levels due to immunoassay interference, later verified as normal using liquid chromatography-tandem mass spectrometry (LC-MS/MS), highlighting the need to consider assay artifacts in diagnosing hyperandrogenism without clinical signs.
February 2019 in “Psychoneuroendocrinology” This study suggests that measuring hair DHEA and testosterone can reliably reflect long-term average hormone levels in women, overcoming daily and menstrual fluctuations seen in salivary hormone assessments.