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GlossaryImmunoassay

laboratory technique detecting molecules using antibodies

An immunoassay is a laboratory technique used to detect and measure specific proteins, hormones, or other molecules in a sample by using antibodies. This method relies on the highly specific binding between an antigen (the target molecule) and an antibody, making it a powerful tool for diagnosing diseases, monitoring biological processes, and conducting research in fields like immunology and biochemistry.

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research Design and Validation of a Sensitive Multisteroid LC-MS/MS Assay for Routine Clinical Use: One-Step Sample Preparation with Phospholipid Removal and Comparison to Immunoassays

9 citations , November 2022 in “International Journal of Molecular Sciences”

The new LC-MS/MS method is more accurate and reliable than traditional immunoassays for measuring steroids in serum.

research Invalidation of a Commercially Available Human 5α-Dihydrotestosterone Immunoassay

13 citations , September 2013 in “Steroids”

The DHT immunoassay is unreliable due to high cross-reactivity with testosterone.

research LC-MS/MS Improves Screening Towards 21-Hydroxylase Deficiency

7 citations , December 2014 in “Gynecological Endocrinology”

LC-MS/MS is more reliable than immunoassays for diagnosing 21-hydroxylase deficiency.

research Measurement of Cortisol in Human Hair as a Biomarker of Systemic Exposure

567 citations , October 2007 in “Clinical and investigative medicine”

Hair cortisol can be used to measure long-term stress exposure.

research Revisiting Hyper- and Hypo-Androgenism by Tandem Mass Spectrometry

27 citations , April 2013 in “Reviews in endocrine and metabolic disorders”

The document concludes that using LC-MS/MS for measuring androgens is more accurate than older methods, but it needs careful validation and standardized references to be most effective.

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community Liquid Chromatography–Mass Spectrometry (LC-MS) VS Enzyme-Linked Immunosorbent Assay (ELISA)

in Research/Science 3 upvotes 4 months ago

Finasteride and Nizoral shampoo with Ketoconazole are used for hair loss treatment. Liquid Chromatography–Mass Spectrometry (LC-MS) is recommended over Enzyme-Linked Immunosorbent Assay (ELISA) for more accurate DHT testing.

community Dutasteride isn’t working confirmed by test.

in Finasteride/Dutasteride 40 upvotes 7 months ago

Dutasteride isn't effectively reducing DHT levels, prompting a switch back to finasteride. The user questions the reliability of their DHT test results due to hair loss concerns.

community Feel like I'm a reasonably good responder

in Is this regrowth? 274 upvotes 1 year ago

The user "OP" reported significant hair regrowth using oral finasteride, oral and topical minoxidil, ketoconazole shampoo, and derma stamping. OP experienced initial shedding but no other side effects.

community High DHT with finasteride. Help!

in Finasteride/Dutasteride 7 upvotes 1 year ago

Despite using 5% minoxidil, 0.1% finasteride, and other treatments like microneedling, MK-677, and Cialis, hair loss continues with high testosterone and DHT levels. Considering oral dutasteride but concerned about further increasing testosterone levels.