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Germ-free rats need biotin for growth and have different vitamin metabolism compared to regular rats.

Methotrexate treats various disorders by inhibiting DNA synthesis, but careful monitoring is needed to avoid toxicity and manage side effects.

Aminopterin strongly suppresses epithelial tissues and can treat certain skin conditions but has significant side effects.

Aminopterin effectively improves skin conditions but has toxic side effects that need careful monitoring.

Chemotherapy can cause skin problems like hair loss, mouth sores, and skin darkening, and recognizing these can affect treatment decisions.