Filtration of Transgenic Sheep Skin Fibroblasts with KAP6.1-GFP-Polymerized Spider Dragline Silk Protein Gene (4S)

This study successfully established a transgenic sheep fibroblast cell line containing the spider dragline silk protein gene, aiming to express this gene in sheep hair follicles through nuclear transplantation. The researchers constructed an eukaryotic expression vector, pIRES2-EGFP-4S, and transfected sheep fibroblasts using a cationic liposome method. The transgenic cells were filtered using G418, and their biological characteristics, such as cellular morphology, growth curve, and plating efficiency, were found to be similar to normal fibroblasts. PCR analysis confirmed the integration of the vector into the sheep genome, laying the groundwork for future development of transgenic sheep expressing spider silk protein in their hair follicles.