Filtration of Transgenic Sheep Skin Fibroblasts with KAP6.1-GFP-Polymerized Spider Dragline Silk Protein Gene (4S)

January 2011 in “ Zhongguo nongye Kexue ”

AN Tie-zhu

transgenic sheep fibroblast spider dragline silk protein hair follicles nuclear transplantation eukaryotic expression vector pIRES2-EGFP-4S cationic liposome G418 PCR analysis

Studysummary This study successfully established a transgenic sheep fibroblast cell line expressing the spider dragline silk protein gene, laying groundwork for developing transgenic sheep with this capability in hair follicles.

Automatically generated from the study's abstract, not written by a person, and not a review of the full paper. Not medical advice or a treatment recommendation. Read the original study, and consult a qualified healthcare professional before changing treatment. Full disclaimer ·

View this study on en.cnki.com.cn →

Discuss this study in the Community →

Similar Research

5 / 1000+ results

research Screening of Sheep Fibroblast Cell Line with the Spider Dragline Silk Protein Gene

January 2009 in “Xumu shouyi xuebao”

In this study, researchers successfully established a stable transgenic sheep fibroblast cell line containing an artificially synthesized spider dragline silk protein gene.

research Production of artificial synthetic spidroin gene 4S-transgenic cloned sheep embryos using somatic cell nuclear transfer

16 citations , March 2020 in “Animal Biotechnology”

This research explored methods to obtain transgenic sheep embryos expressing synthetic spider silk genes in hair follicles using somatic cell nuclear transfer, with successful in-vitro development observed.

research Stable transfection and identification of a hair follicle-specific expression vector of IGFBP-5 in goat fetal fibroblasts

6 citations , January 2014 in “Genetics and Molecular Research”

This study constructed a hair follicle-specific expression vector for IGFBP-5 in Inner Mongolia Cashmere goat cells, allowing for future functional genetic analyses and potential use in nuclear transfer.

research Construction and Verification of Recombinant Follicle-specific Expression Vector

January 2011 in “Anhui nongye kexue”

This study reports that the recombinant expression vector pcDNA3.1-KK demonstrates specific expression in the skin of newborn mice.

research Study on the Activity of Sheep High-sulfur Keratin Promoter

January 2009 in “China Animal Husbandry & Veterinary Medicine”

This study found that the sheep high-sulfur keratin promoter B2C initiated GFP expression in sheep fibroblasts but remained inactive in mouse embryos.