Construction and Verification of Recombinant Follicle-Specific Expression Vector
January 2011
in “
Anhui nongye kexue
”
Studysummary This study reports that the recombinant expression vector pcDNA3.1-KK demonstrates specific expression in the skin of newborn mice.
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The study aimed to construct a recombinant follicle-specific expression vector to express the keratin type II intermediate filament gene (KIF II-9) specifically in hair follicles. Researchers designed primers based on the ovine keratin associated protein 6-1 (KAP6-1) gene to amplify its 5' regulating sequence. They constructed the vector pcDNA3.1-KK by combining this sequence with KIF II-9 cDNA and a CMV promoter-less pcDNA3.1. The vector's specificity was tested by injecting it into the skin of newborn mice, and results showed that it efficiently directed the transcription of KIF II-9 in the skin, confirming its specific expression.