Construction and Verification of Recombinant Follicle-Specific Expression Vector

    January 2011 in “ Anhui nongye kexue ”
    YU Yong-sheng
    TLDR The vector successfully directed specific gene expression in hair follicles.
    The study aimed to construct a recombinant follicle-specific expression vector to express the keratin type II intermediate filament gene (KIF II-9) specifically in hair follicles. Researchers designed primers based on the ovine keratin associated protein 6-1 (KAP6-1) gene to amplify its 5' regulating sequence. They constructed the vector pcDNA3.1-KK by combining this sequence with KIF II-9 cDNA and a CMV promoter-less pcDNA3.1. The vector's specificity was tested by injecting it into the skin of newborn mice, and results showed that it efficiently directed the transcription of KIF II-9 in the skin, confirming its specific expression.
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